| Registro completo |
| Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
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| País: |
Brazil
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| Título: |
Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
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| Autores: |
Carvalho,R.
Passos,L.M.F.
Oliveira,A.M.
Henry,M.
Martins,A.S.
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| Data: |
2000-08-01
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| Ano: |
2000
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| Palavras-chave: |
Equine
Semen
Embryo
EHV-1
EHV-4
PCR
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| Resumo: |
The genome of one equine embryo and three equine semen specimens collected from a Brazilian farm were tested by polymerase chain reaction (PCR) for the presence of EHV-1 and EHV-4-specific timidine kinase (TK) sequences. The PCR detected specific EHV-1 TK gene sequences in all samples tested. The peripheral blood leukocytes (PBL) of the embryo donor mare also was amplified by EHV-1 TK primers. Infectious virus was not recovered from any specimens. The animals did not show any clinical signal of EHV-1 or EHV-4 infections. EHV-4 was not detected in the studied specimens. The results indicate that PCR was more sensitive than virus isolation in cell culture for detecting EHV-1 in semen of carrier horses.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400002
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| Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
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| Relação: |
10.1590/S0102-09352000000400002
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| Formato: |
text/html
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| Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.52 n.4 2000
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| Direitos: |
info:eu-repo/semantics/openAccess
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